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Subfractionation of Low-Density Lipoprotein (LDL) - Details

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Protocols » Listing Details
ID:1585
Title: Subfractionation of Low-Density Lipoprotein (LDL) - http://www.axis-shield.com/densityhome/optiprep/M11.pdf
Description:In the routine method described in this protocol, chylomicron-free plasma is adjusted to 12% (w/v) iodixanol and the sample, essentially fills an approx 3 ml tube for a near-vertical rotor. During the centrifugation VLDL, LDL and HDL particles and also plasma proteins migrate from all parts of the sample to their final buoyant density banding position in the self-forming density gradient.
Category:Cell Organelle Protocols: Subcellular Fractionation Protocols
Link Owner:
Date Added:December 04, 2007 05:34:42 AM
Number Hits:30
 

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