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Fractionation of Vacuolar and Subvacuolar vesicles and Vacuole and Cytoplasm-to-Vacuole Targeting - Details

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Protocols » Listing Details
ID:1580
Title: Fractionation of Vacuolar and Subvacuolar vesicles and Vacuole and Cytoplasm-to-Vacuole Targeting - http://www.axis-shield.com/densityhome/optiprep/S29.pdf
Description:Fractionation of (a) vacuolar and subvacuolar vesicles and (b) vacuole and cytoplasm-to-vacuole targeting (Cvt) vesicles from yeast spheroplasts in a pre-formed discontinuous iodixanol gradients. Protocol includes: Formation of yeast spheroplasts; Isolation and vesiculation of the vacuoles; Separation of the vacuolar and subvacuolar vesicles; Separation of vacuoles and Cvt vesicles from a yeast spheroplast lysate.
Category:Cell Organelle Protocols: Subcellular Fractionation Protocols
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Date Added:December 03, 2007 11:56:24 AM
Number Hits:67
 

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