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Western Blot Analysis of Sub-Cellular Fractionated Samples Using the Odyssey Infrared Imaging System - Details

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Protocols » Listing Details
ID:1576
Title: Western Blot Analysis of Sub-Cellular Fractionated Samples Using the Odyssey Infrared Imaging System - http://www.natureprotocols.com/2006/09/15/western_blot_analysis_of_subce.php
Description:This protocol describes nuclear and cytoplasmic fractionation of tissue culture cells and a method for Western blot detection of proteins using the Odyssey Infrared Imaging System. This protocol was used to detect expression of the "small" Tap protein in 293T, HeLa and COS cells. The Odyssey system has several advantages over the more widely used chemiluminescent detection methods.
Category:Cell Organelle Protocols: Subcellular Fractionation Protocols
Link Owner:
Date Added:December 03, 2007 11:46:57 AM
Number Hits:105
 

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