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Imaging Actin in Tissue Slices from Transgenic Mouse Brain Protocol - Details

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Protocols » Listing Details
ID:1524
Title: Imaging Actin in Tissue Slices from Transgenic Mouse Brain Protocol - http://www.cshprotocols.org/cgi/content/extract/2007/3/pdb.ip28
Description:The same GFP-tagged actin construct used in cell transfection experiments has been used to produce transgenic mice. Transgenic animals allow the imaging of brain tissue in the intact animal, as acutely cut slices or as organotypic slice cultures. They also serve as a source of cells for imaging neurons at high resolution in dispersed low-density cell culture. In contrast to cells transfected in culture, where the level of actin-GFP expression in neurons varies considerably, transgenic mice...
Category:Cytoskeleton Protocols: Actin Myosin Protocols
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Date Added:December 01, 2007 02:00:20 PM
Number Hits:42
 

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