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Analysis of Mitochondrial Membrane Potential with the Sensitive Fluorescent Probe JC-1 - Details

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Protocols » Listing Details
ID:1514
Title: Analysis of Mitochondrial Membrane Potential with the Sensitive Fluorescent Probe JC-1 - http://www.cyto.purdue.edu/flowcyt/research/cytotech/apopto/data/cossar1/cossariz.htm
Description:The technique of JC-1 staining has been developed with the intent to detect DY in intact, viable cells. For this purpose JC-1 acts as a marker of mitochondrial activity, since the formation of J-aggregates, which give red emission, is reversible. Cells with high DY are those forming J-aggregates, thus showing high red fluorescence. On the other hand, cells with low DY are those in which JC-1 maintains (or re-acquire) monomeric form, thus showing only green fluorescence.
Category:Cell Organelle Protocols: Mitochondria Protocols
Link Owner:
Date Added:December 01, 2007 04:46:27 AM
Number Hits:91
 

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