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Synchronization of Mammalian Cell Cultures in Mitosis Using Selective Detachment Protocol - Details

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Protocols » Listing Details
ID:1494
Title: Synchronization of Mammalian Cell Cultures in Mitosis Using Selective Detachment Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4489
Description:Protocol describes a method for synchronizing monolayer cells in mitosis using selective detachment from their substrate. During mitosis, cells become spherical, causing them to become more loosely attached to their substrate. The "rounded up" cells are selectively detached by tapping the culture flask, resulting in a population in which as many as 90-98% of the cells are in mitosis. The drug nocodazole is used to increase the percentage of cells undergoing mitosis before detachment is performed
Category:Cell Biology and Cell Culture: Cell Cycle Protocols: Mitosis Protocols
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Date Added:November 30, 2007 02:58:10 PM
Number Hits:9
 

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