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Studying Mitosis in Cultured Mammalian Cells Prtocol - Details

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Protocols » Listing Details
ID:1493
Title: Studying Mitosis in Cultured Mammalian Cells Prtocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4674
Description:Protocol describes methods for maintaining healthy, dividing mammalian cells in culture and during imaging, when mitosis can be examined. Rose chambers are preferable for observation and microinjection of living mitotic cells, but slide/coverslip preparations are easy to make and do not require any special equipment. Another inexpensive and easy-to-use alternative is to grow cells in a culture dish with a glass bottom. Such dishes are suitable for microinjection experiments.
Category:Cell Biology and Cell Culture: Cell Cycle Protocols: Mitosis Protocols
Link Owner:
Date Added:November 30, 2007 02:56:19 PM
Number Hits:10
 

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