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G1/S Phase Synchronization Using Mimosine Arrest Protocol - Details

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Protocols » Listing Details
ID:1490
Title: G1/S Phase Synchronization Using Mimosine Arrest Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4488
Description:This protocol uses the plant amino acid mimosine as a G1/S synchronizing agent. Cells are first treated with excess thymidine to accumulate the majority of them at G1/S; however, some cells will have stopped growth within the S phase. Thymidine is then removed to allow all the cells to proceed completely through the S phase. Mimosine is then added to arrest the cells at the G1/S border. When mimosine is removed, cells will begin to enter S phase within about 1 hour.
Category:Cell Biology and Cell Culture: Cell Cycle Protocols: G Phase Protocols
Link Owner:
Date Added:November 30, 2007 02:50:18 PM
Number Hits:6
 

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