Special Feature

User Panel

My Panel

My Panel

Bookmark Science Articles

Recent News
Bookmark / Share This Science Site

Send Us Your Protocols

G1 Synchronization of CHO Cells by Isoleucine Deprivation Protocol - Details

Link Details 

Protocols » Listing Details
ID:1488
Title: G1 Synchronization of CHO Cells by Isoleucine Deprivation Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4486
Description:This protocol provides a method for the synchronization of a monolayer culture of CHO cells in G1 using isoleucine deprivation. Since CHO cells can also be adapted to grow in suspension culture, this procedure can be used to obtain larger quantities of cells. When isoleucine is replaced, the cells resume growth and begin to enter S phase ~4 hours later. This method arrests almost 100% of the CHO cells in G1, and upon reversal, leads to rapid recovery of cell growth and very high cell viability.
Category:Cell Biology and Cell Culture: Cell Cycle Protocols: G Phase Protocols
Link Owner:
Date Added:November 30, 2007 02:48:14 PM
Number Hits:57
 

Protocols Tags

  • amounts Protocols
  • amplification Protocols
  • analysis Protocols
  • assay Protocols
  • based Protocols
  • buffer Protocols
  • ca2 Protocols
  • calcium Protocols
  • calculator Protocols
  • cell Protocols
  • cells Protocols
  • chromatin Protocols
  • cloning Protocols
  • cycle Protocols
  • cyp2c9 Protocols
  • cytometry Protocols
  • describes Protocols
  • dewhurst Protocols
  • display Protocols
  • dna Protocols
  • electroporation Protocols
  • enzymes Protocols
  • free Protocols
  • guide Protocols
  • hybridization Protocols
  • immunoprecipitation Protocols
  • includes Protocols
  • lab Protocols
  • microdissected Protocols
  • molecular Protocols
  • pcr Protocols
  • phage Protocols
  • preparation Protocols
  • processing Protocols
  • promega Protocols
  • quantitative Protocols
  • raw Protocols
  • reactions Protocols
  • rna Protocols
  • samples Protocols
  • sensitive Protocols
  • studies Protocols
  • taqman Protocols
  • template Protocols
  • tool Protocols
  • transcription Protocols
  • vitro Protocols
  • 0 Protocols
  • u2os Protocols
  • yeast Protocols


    •  

    Molecular Station Menu

    Welcome to Molecular Station!

    You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!

    Already registered? Login now below.

    User Name:

    Password:

    Already registered and Forgot your password? Click below to recover it.

    Recover Lost Password

    Home
    Features

    Protocols

    DNA Forum

    Science Forum

    DNA Forum
    Biology Forum