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Tetracycline as Regulator of Inducible Gene Expression Protocol - Details

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Protocols » Listing Details
ID:1485
Title: Tetracycline as Regulator of Inducible Gene Expression Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3986
Description:Protocol uses an autoregulatory system in which the transcriptional trans-activator tTA drives its own expression and that of a target gene. The first stage of the procedure describes how to generate stable lines of NIH-3T3 cells that express either tTA alone or tTA and the tetracycline-regulated target gene.
Category:Transfection Protocols: Stable Transfection Protocols
Link Owner:
Date Added:November 30, 2007 02:27:52 PM
Number Hits:63
 

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