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Establishment of Stable Transfectant of CHO Lec Cells Protocol - Details

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Protocols » Listing Details
ID:1484
Title: Establishment of Stable Transfectant of CHO Lec Cells Protocol - http://www.cbrinstitute.org/labs/springer/protocols/jun_CHOlec.html
Description:CHO Lec3.2.8.1 cells have 4 independent mutations in the N and O glycosylation pathways. When cultured with alpha-glucosidase I inhibitor N-butyl-deoxynojirimycin, glycoproteins produced in CHO Lec3.2.8.1 cells are completely susceptible to Endo H digestion. Endo H cleaves chitobiose, leaving a single N-linked N-acetylglucosamine per site which is ideal for maintenance of protein solubility and special carb-protein interactions, such as between the first N-acetyl glucosamine residue and tryp.
Category:Transfection Protocols: Stable Transfection Protocols
Link Owner:
Date Added:November 30, 2007 02:26:20 PM
Number Hits:89
 

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