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Transient Transfection Into 293T Cells Protocol - Details

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Protocols » Listing Details
ID:1480
Title: Transient Transfection Into 293T Cells Protocol - http://www.cbrinstitute.org/labs/springer/protocols/jun_293T.html
Description:Transient transfection into 293T cells is a convenient way to overexpress and obtain both cellular and extracellular (secreted or membrane) proteins. 293 is a human renal epithelial cell line which is transformed by adenovirus E1A gene product. 293T is a derivative which also express SV40 large T antigen, allowing episomal replication of plasmids containing the SV40 origin and early promoter region. They (both) have the unusual property of being highly transfectable.
Category:Transfection Protocols: Transient Transfection Protocols
Link Owner:
Date Added:November 30, 2007 07:47:58 AM
Number Hits:147
 

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