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Transfecting and Plating RAW 264.7 Cells with Lipofectamine 2000 Protocol - Details

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Protocols » Listing Details
ID:1469
Title: Transfecting and Plating RAW 264.7 Cells with Lipofectamine 2000 Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000182.pdf?pid=PP00000182
Description:The following procedure is for simultaneous transfection and plating of RAW 264.7 cells. This protocol results in approximately 50% to 70% cell viability, and of those viable cells, 20% to 40% are transfected when using pEYFP-N1 from Clontech. Include: Procedure for Splitting Cells before Transfection; Procedure for Preparing Lipofectamine 2000 and DNA; Preparation of RAW 264.7 Cells for Transfection.
Category:Transfection Protocols
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Date Added:November 30, 2007 06:02:24 AM
Number Hits:719
 

 

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