Special Feature

User Panel

My Panel

My Panel

Bookmark Science Articles

Recent News
Bookmark / Share This Science Site

Send Us Your Protocols

Transfecting and Plating RAW 264.7 Cells with Lipofectamine 2000 Protocol - Details

Link Details 

Protocols » Listing Details
ID:1469
Title: Transfecting and Plating RAW 264.7 Cells with Lipofectamine 2000 Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000182.pdf?pid=PP00000182
Description:The following procedure is for simultaneous transfection and plating of RAW 264.7 cells. This protocol results in approximately 50% to 70% cell viability, and of those viable cells, 20% to 40% are transfected when using pEYFP-N1 from Clontech. Include: Procedure for Splitting Cells before Transfection; Procedure for Preparing Lipofectamine 2000 and DNA; Preparation of RAW 264.7 Cells for Transfection.
Category:Transfection Protocols
Link Owner:
Date Added:November 30, 2007 06:02:24 AM
Number Hits:96
 

Protocols Tags

  • agrobacterium Protocols
  • amplification Protocols
  • based Protocols
  • beads Protocols
  • cells Protocols
  • chambers Protocols
  • clearing Protocols
  • coli Protocols
  • colonies Protocols
  • culture Protocols
  • describes Protocols
  • development Protocols
  • dna Protocols
  • drosophila Protocols
  • during Protocols
  • electroporation Protocols
  • elegans Protocols
  • embryonic Protocols
  • embryos Protocols
  • first Protocols
  • fluorescence Protocols
  • fly Protocols
  • food Protocols
  • gal Protocols
  • lioac Protocols
  • liver Protocols
  • methylated Protocols
  • microscopy Protocols
  • mouse Protocols
  • neurons Protocols
  • non Protocols
  • p450 Protocols
  • pcr Protocols
  • photon Protocols
  • prep Protocols
  • primary Protocols
  • procedure Protocols
  • secinh3 Protocols
  • state Protocols
  • system Protocols
  • techniques Protocols
  • than Protocols
  • that Protocols
  • transfer Protocols
  • transformation Protocols
  • two Protocols
  • uterine Protocols
  • vector Protocols
  • without Protocols
  • yeast Protocols


    •  

    Molecular Station Menu

    Welcome to Molecular Station!

    You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!

    Already registered? Login now below.

    User Name:

    Password:

    Already registered and Forgot your password? Click below to recover it.

    Recover Lost Password

    Home
    Features

    Protocols

    DNA Forum

    Science Forum

    DNA Forum
    Biology Forum