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DNA Transfection Using Polybrene Protocol - Details

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Protocols » Listing Details
ID:1468
Title: DNA Transfection Using Polybrene Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3875
Description:Polybrene and DMSO can be used to achieve stable transformation of several types of cells by plasmid DNA. The yield of transformants is up to 15-fold greater with Polybrene than with calcium phosphate-DNA coprecipitation. However, there is no difference between the two methods in the efficiency of transformation of cells by high-molecular-weight DNA.
Category:Transfection Protocols
Link Owner:
Date Added:November 30, 2007 05:56:25 AM
Number Hits:35
 

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