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Transfection of Hippocampal Neurons with Plasmid DNA Using Calcium Phosphate Coprecipitation - Details

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Protocols » Listing Details
ID:1465
Title: Transfection of Hippocampal Neurons with Plasmid DNA Using Calcium Phosphate Coprecipitation - http://www.cshprotocols.org/cgi/content/abstract/2006/1/pdb.prot4445
Description:This protocol describes transfection of plasmid DNA into primary hippocampal neurons using DNA/calcium-phosphate (CaPO4) coprecipitation. The precise pH of the transfection medium and the incubation time of cells with the coprecipitate are critical for reproducible and efficient transfection. Once these parameters are optimized for a given plasmid, the method is easily adapted for transfection of other established cell lines.
Category:Transfection Protocols: Calcium Phosphate Transfection Protocols
Link Owner:
Date Added:November 30, 2007 05:51:48 AM
Number Hits:44
 

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