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Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid - Details

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Protocols » Listing Details
ID:1464
Title: Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4664
Description:This protocol describes two transfection methods for expressing GFP-tagged actin in primary neurons. The lipid reagent DOTAP (Roche Diagnostics) method produces actin-GFP-expressing hippocampal neurons that survive well during long periods in culture. The calcium phosphate method can be used to transfect neurons that have already been growing on coverslips in vitro. Transfected cells suitable for imaging can be obtained in cultures up to 15 days in vitro.
Category:Transfection Protocols: Calcium Phosphate Transfection Protocols
Link Owner:
Date Added:November 30, 2007 05:50:50 AM
Number Hits:34
 

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