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Calcium-phosphate-mediated Transfection of Eukaryotic Cells with Plasmid DNAs - Details

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Protocols » Listing Details
ID:1463
Title: Calcium-phosphate-mediated Transfection of Eukaryotic Cells with Plasmid DNAs - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3871
Description:Calcium phosphate forms an insoluble precipitate with DNA, which attaches to the cell surface and is taken into the cells by endocytosis. The protocol is easily adapted for use with other types of cells, both adherent and nonadherent. This protocol is a modified version of a method published by Jordan et al. (1996) who rigorously optimized calcium-phosphate-based transfection methods for Chinese hamster ovary cells and the 293 line of human embryonic kidney cells.
Category:Transfection Protocols: Calcium Phosphate Transfection Protocols
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Date Added:November 30, 2007 05:49:42 AM
Number Hits:767
 

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