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Calcium Phosphate Transfection Method - Details

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Protocols » Listing Details
ID:1461
Title: Calcium Phosphate Transfection Method - http://www.flemingtonlab.com/Protocols/CalciumPhosphateTransf.pdf
Description:This calcium phosphate transfection method works best in cell lines that are 1) highly transformed and 2) adherent (Hela, U2OS, SAOS2, AdAH, NPC-KT and obtain from 20% to 100% transfection efficiency depending on the cell line). Works well for transient experiments but precautions should be used in the design and interpretation of experiments based on the discussion below. Also works very well for generating stable cell lines. This method is quite sensitive to the amount of input plasmid.
Category:Transfection Protocols: Calcium Phosphate Transfection Protocols
Link Owner:
Date Added:November 30, 2007 05:45:25 AM
Number Hits:1111
 

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