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Transfection of Bone Marrow-Derived Mast Cells for Transcription Factor Luciferase Reporter Assays - Details

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Protocols » Listing Details
ID:1459
Title: Transfection of Bone Marrow-Derived Mast Cells for Transcription Factor Luciferase Reporter Assays - http://www.natureprotocols.com/2006/09/12/transfection_of_bone_marrowder_1.php
Description:Transfection of primary leukocytes has traditionally been a challenging but much desired protocol. It allows not only the analysis of cells in a more natural state to a cell line system, it enables the direct comparison of, for e.g. transcriptional activity using luciferase reporters, in immune cells taken from genetically-altered mice. In addition, importantly it allows for "rescue experiments" in knockout cells & the ability to over-express or reconstitute wild-type and/or mutated constructs.
Category:Reporter Gene Assays: Luciferase Assay Protocols
Link Owner:
Date Added:November 29, 2007 10:55:30 AM
Number Hits:61
 

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