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Split Luciferase Complementation Assay for Studying Interaction of Proteins X and Y in Cells - Details

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Protocols » Listing Details
ID:1457
Title: Split Luciferase Complementation Assay for Studying Interaction of Proteins X and Y in Cells - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4596
Description:Protocol describes a split luciferase complementation assay used to study the interaction of proteins in cells. In the split protein strategy, a single reporter protein/enzyme (firefly luciferase [Fluc]) is cleaved into amino-terminal and carboxy-terminal halves; each half is fused to one of two interacting proteins, X & Y. Physical interactions between the two proteins reconstitute the functional reporter protein, leading to enzymatic activities that can be measured by in vitro or in vivo assay
Category:Reporter Gene Assays: Luciferase Assay Protocols
Link Owner:
Date Added:November 29, 2007 10:50:33 AM
Number Hits:118
 

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