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Assay for Luciferase in Extracts of Mammalian Cells Protocol - Details

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Protocols » Listing Details
ID:1450
Title: Assay for Luciferase in Extracts of Mammalian Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3642
Description:In this protocol, cells transfected with a luciferase reporter plasmid are lysed in a detergent-containing buffer. Luciferase in the extract catalyzes an oxidation reaction in which D-luciferin is converted to oxyluciferin, with production of light at 556 nm that can be quantified in a luminometer.
Category:Reporter Gene Assays: Luciferase Assay Protocols
Link Owner:
Date Added:November 29, 2007 10:02:14 AM
Number Hits:1094
 

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