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Probing Protein Interactions Using GFP and FRET Protocol - Details

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Protocols » Listing Details
ID:1442
Title: Probing Protein Interactions Using GFP and FRET Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3893
Description:In preparation for FLIM-FRET analysis, the appropriate donor and acceptor components must be introduced into live or fixed cells. The method of introduction depends on the nature of the components and the state of the cells. For example, plasmid DNAs encoding a protein of interest fused to a variant of GFP may be introduced into live cells by transfection or microinjection, whereas labeled antibodies are delivered by microinjection.
Category:Reporter Gene Assays: GFP Assay Protocols
Link Owner:
Date Added:November 29, 2007 09:47:07 AM
Number Hits:24
 

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