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Constructing and Expressing GFP Fusion Proteins - Details

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Protocols » Listing Details
ID:1436
Title: Constructing and Expressing GFP Fusion Proteins - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4649
Description:The following protocol can be used for the development of stable cell lines expressing GFP fusion proteins. Although optimal transfection procedures (e.g., calcium phosphate, electroporation, or FuGENE 6 [Roche Applied Science]) vary depending on cell type, this general transfection procedure has been successful for stable transfection of HeLa, A-431, U2OS, BHK, and HT1080 cells.
Category:Reporter Gene Assays: GFP Assay Protocols
Link Owner:
Date Added:November 29, 2007 09:37:21 AM
Number Hits:513
 

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