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Assay for Phage Containing the Beta-galactosidase Gene - Details

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Protocols » Listing Details
ID:1425
Title: Assay for Phage Containing the Beta-galactosidase Gene - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda7.html
Description:This assay is used when working with phage vectors carrying the beta-gal gene. If the cloning event disrupts a normally functional copy of the gene in the vector the resulting plaques would appear clear in the assay. If the phages contain a functional beta-gal gene they will form blue rings around their plaques. Any strain which is not an overproducer of beta-gal will work as indicator host bacteria; a single chromosomal copy of the gene is not a problem.
Category:Reporter Gene Assays: B-Galactosidase Assay Protocols
Link Owner:
Date Added:November 29, 2007 09:14:59 AM
Number Hits:93
 

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