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Quantitative GUS Activity Assay in Intact Plant Tissue Protocol - Details

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Protocols » Listing Details
ID:1422
Title: Quantitative GUS Activity Assay in Intact Plant Tissue Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4688
Description:The activity of ß-glucuronidase (GUS) can be accurately determined in intact plant tissue using 4-methylumbelliferyl ß-D-glucuronide (4-MUG) as a substrate. Upon hydrolysis by GUS, the fluorochrome 4-methyl umbelliferone (4-MU) is produced. This method is based on the permeability of both 4-MUG and 4-MU through plant tissue. It consists of incubation of the tissue with the reagent and quantification of the fluorescence emitted by 4-MU in the solution. GUS activity in each sample can be...
Category:Plant Biology Protocols: Plant Proteins, Peptides and Antigens
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Date Added:November 29, 2007 03:51:30 AM
Number Hits:138
 

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