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Fractionation of Maize Embryo Proteins for 2-D Gel Electrophoresis Using Multicompartment Electrolyz - Details

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Protocols » Listing Details
ID:1421
Title: Fractionation of Maize Embryo Proteins for 2-D Gel Electrophoresis Using Multicompartment Electrolyz - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4235
Description:Protocol describes a method for performing isoelectric fractionation of a maize embryo sample using a multicompartment electrolyzer(MCE). This prefractionation of proteins having pIs within a certain pH interval is essential for allowing high loads of protein to be resolved on narrow and ultra-narrow immobilized pH gradients used in 2D electrophoresis. The isoelectric membranes in the MCE act like isoelectric traps capturing all the protein species having pIs encompassing the pI value of each...
Category:Plant Biology Protocols: Plant Proteins, Peptides and Antigens
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Date Added:November 29, 2007 03:43:10 AM
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