Special Feature

User Panel

My Panel

My Panel

Bookmark Science Articles

Recent News
Bookmark / Share This Science Site

Send Us Your Protocols

AFLP For Positional Cloning - Details

Link Details 

Protocols » Listing Details
ID:1395
Title: AFLP For Positional Cloning - http://www-ciwdpb.stanford.edu/publications/methods/aflp.html
Description:AFLP was designed as a highly sensitive method for DNA fingerprinting to be used in a variety of fields. We are using this technology to generate DNA based markers for cloning genes involved in phototropic responses in higher plants that have only been identified genetically by mutant phenotype. Protocol includes: Generate polymorphic recombinant F2 (or F3) population; Isolate genomic DNA; Restriction of DNA; Ligation of adapters; Pre-amplification of template DNA; AFLP-PCR; etc.
Category:Plant Biology Protocols: Plant Genetics Protocols
Link Owner:
Date Added:November 28, 2007 05:42:06 AM
Number Hits:73
 

Protocols Tags

  • assay Protocols
  • caged Protocols
  • carries Protocols
  • cell Protocols
  • cells Protocols
  • chromatography Protocols
  • column Protocols
  • culture Protocols
  • deubiquitinating Protocols
  • development Protocols
  • dna Protocols
  • double Protocols
  • drosophila Protocols
  • dual Protocols
  • elegans Protocols
  • enzymes Protocols
  • fish Protocols
  • fixation Protocols
  • hybridization Protocols
  • imaging Protocols
  • includes Protocols
  • labeling Protocols
  • low Protocols
  • luciferase Protocols
  • may Protocols
  • neurons Protocols
  • obtained Protocols
  • optimized Protocols
  • pdf Protocols
  • peptide Protocols
  • peptides Protocols
  • preparation Protocols
  • primary Protocols
  • protein Protocols
  • proteins Protocols
  • quality Protocols
  • reporter Protocols
  • samples Protocols
  • single Protocols
  • sscp Protocols
  • synthesis Protocols
  • system Protocols
  • target Protocols
  • techniques Protocols
  • that Protocols
  • time Protocols
  • tissue Protocols
  • tissues Protocols
  • troubleshooting Protocols
  • yeast Protocols


    •  

    Molecular Station Menu

    Welcome to Molecular Station!

    You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!

    Already registered? Login now below.

    User Name:

    Password:

    Already registered and Forgot your password? Click below to recover it.

    Recover Lost Password

    Home
    Features

    Protocols

    DNA Forum

    Science Forum

    DNA Forum
    Biology Forum