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A Method for Isolation of Chloroplast DNA and Mitochondrial DNA from Sunflower - Details

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Protocols » Listing Details
ID:1369
Title: A Method for Isolation of Chloroplast DNA and Mitochondrial DNA from Sunflower - http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb16/16183-2.pdf
Description:The protocol includes: organelle isolation, deoxyribonuclease treatment, lysis, deproteinisation and a final DNA purification with sodium dodecyl sulphate and potassium acetate. The organelle DNA yield is 5–10 micrograms per gram of tissue and the DNA is fully restrictable. The technique is inexpensive and appropriate for the isolation of multiple samples of organelle DNA from a small amount of tissue.
Category:Plant Biology Protocols: Plant DNA RNA Isolation Protocols
Link Owner:
Date Added:November 28, 2007 03:39:35 AM
Number Hits:422
 

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