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Photoactivation-Based Labeling and In Vivo Tracking of RNA Molecules in the Nucleus - Details

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Protocols » Listing Details
ID:1368
Title: Photoactivation-Based Labeling and In Vivo Tracking of RNA Molecules in the Nucleus - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4600
Description:This protocol describes a method for observing and measuring the movement of RNA molecules in the nucleus of living mammalian cells. Caged fluorescein-labeled DNA oligonucleotides are introduced into living mammalian cells, where they demonstrably hybridize to complementary RNA. After site-specific photoactivation at desired sites within the cell, the RNA movements away from those sites are followed and digitally recorded using a rapid acquisition microscopy system.
Category:Microscopy Protocols: Live-Cell Imaging Protocols
Link Owner:
Date Added:November 27, 2007 05:34:59 PM
Number Hits:12
 

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