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Live-Cell Imaging of GFP in Plants - Details

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Protocols » Listing Details
ID:1365
Title: Live-Cell Imaging of GFP in Plants - http://www.cshprotocols.org/cgi/content/full/2007/3/pdb.ip31
Description:GFP serves as a molecular marker that can be imaged dynamically in living cells, both in its native form & as a fusion to other proteins. For GFP imaging, plants present the challenge of autofluorescence from chlorophyll, lignified cell walls, vacuolar contents, and other cell materials, all of which can obscure the GFP signal. Maximizing the signal-to-noise ratio is a major concern, and careful consideration should be given to the choice of tissue imaged, GFP expression level, etc.
Category:Microscopy Protocols: Live-Cell Imaging Protocols
Link Owner:
Date Added:November 27, 2007 05:31:02 PM
Number Hits:52
 

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