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Live Single-Cell Fura-2 Measurements to Determine the Intracellular Free Calcium - Details

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Protocols » Listing Details
ID:1364
Title: Live Single-Cell Fura-2 Measurements to Determine the Intracellular Free Calcium - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000188.pdf?pid=PP00000188
Description:This protocol describes a method to assess concentrations of free cytoplasmic calcium, [Ca2+]i, for cultured adherent RAW 264.7 cells in an 8-well coverglass. This objective is accomplished using the Ca2+-sensitive fluorescent dye, fura-2 acetoxymethyl (AM), which permeates cell membranes as an ester and is hydrolyzed in the cell to its Ca2+-sensitive acidic form. Fluorescence for the adherent cells is measured over time with cells that have been washed free of extracellular dye.
Category:Microscopy Protocols: Live-Cell Imaging Protocols
Link Owner:
Date Added:November 27, 2007 05:27:35 PM
Number Hits:149
 

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