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Imaging of Organelle Membrane Systems and Membrane Traffic in Living Cells - Details

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Protocols » Listing Details
ID:1362
Title: Imaging of Organelle Membrane Systems and Membrane Traffic in Living Cells - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4603
Description:Using confocal laser-scanning microscope & GFP fusion proteins in time-lapse imaging to visualize the behavior of organelles and to track membrane-bound transport intermediates that bud off from organelles. Practical issues related to construction & expression of GFP fusion proteins are discussed. Essential for optimizing the brightness and expression levels of GFP fusion proteins so that intracellular membrane-bound structures containing these fusion proteins can be readily visualized.
Category:Microscopy Protocols: Live-Cell Imaging Protocols
Link Owner:
Date Added:November 27, 2007 05:22:19 PM
Number Hits:100
 

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