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Electron Microscopy of the Cytoskeleton of Cultured Cells - Details

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Protocols » Listing Details
ID:1348
Title: Electron Microscopy of the Cytoskeleton of Cultured Cells - http://www.borisylab.northwestern.edu/pages/protocols/electmicrosc.html
Description:Article describe the preparation of cells for correlative electron microscopy after live light microscopic observation of fluorescently labeled cytoskeletal proteins microinjected into the same cells. Since identification of cytoskeletal elements in electron microscopic preparations is an essential part of any correlative study, procedures for immunogold labeling of cytoskeletal components and for myosin S1 decoration of actin filaments are also described.
Category:Microscopy Protocols: Electron Microscopy Protocols
Link Owner:
Date Added:November 27, 2007 04:40:52 PM
Number Hits:35
 

 

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