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Live Cell Spinning Disk Confocal Fluore Imaging of Cells- Colocalization of Fluorescent Protein Tags - Details

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Protocols » Listing Details
ID:1325
Title: Live Cell Spinning Disk Confocal Fluore Imaging of Cells- Colocalization of Fluorescent Protein Tags - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000143.pdf?pid=PP00000143
Description:Protocol describes the acquisition and processing of confocal fluorescent and bright field images of live cells, expressing cyan fluorescent protein(CFP) and/or yellow fluorescent protein (YFP), with a spinning disk confocal head on a Zeiss Axiovert 200 M microscope. This procedure is used to help determine if N- or Cterminal tagging of signaling molecules alters the steady state localization pattern of the signaling protein in question.
Category:Microscopy Protocols: Confocal Microscopy Protocols
Link Owner:
Date Added:November 27, 2007 05:42:45 AM
Number Hits:43
 

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