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Sumoylation and Desumoylation Assays for a Chromatin-Remodelling Complex In Vivo and In Vitro - Details

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Protocols » Listing Details
ID:1319
Title: Sumoylation and Desumoylation Assays for a Chromatin-Remodelling Complex In Vivo and In Vitro - http://www.natureprotocols.com/2006/11/09/sumoylation_and_desumoylation.php
Description:Small ubiquitin-like modifier (SUMO) is a small molecule, but has a variety of regulatory functions in cells. SUMO modification is involved in transcriptional regulation, subcellular localization, and protein-protein interactions. SUMO conjugation requires sequential E1-dependent activation, E2-dependent conjugation, and E3-dependent ligation steps. Protocol includes: In vivo and in vitro SUMOylation assay and deSUMOylation assay.
Category:Cell Biology and Cell Culture: In Vitro Reconstitution Protocols
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Date Added:November 27, 2007 03:03:53 AM
Number Hits:27
 

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