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Protocol: Purification of In Vitro Synthesized mRNA with Microcon or Centricon Centrifugal Filters - Details

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Protocols » Listing Details
ID:1317
Title: Protocol: Purification of In Vitro Synthesized mRNA with Microcon or Centricon Centrifugal Filters - http://www.millipore.com/techpublications/tech1/6dlk4v
Description:In vitro transcription reactions employing T3, T7 or SP6 phage-encoded RNA polymerases are widely used to synthesize RNA from recombinant vectors containing appropriate promoters. Production of large amounts of specific RNA is valuable in the preparation of hybridization probes and in vitro translation studies; in the synthesis of ribozymes, rRNA, SRP, antisense RNA and substrates for RNA splicing; and in RNA-protein interaction studies.
Category:Cell Biology and Cell Culture: In Vitro Reconstitution Protocols
Link Owner:
Date Added:November 27, 2007 02:58:37 AM
Number Hits:44
 

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