Bioinformatics, Protocols, DNA RNA Protein Proteomics
home > protocol-links > detail.php
 |
|---|
You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!
Already registered? Login now below.
Already registered and Forgot your password? Click below to recover it.
Join now - it's fast and free!
Molecular Station is THE largest network of researchers, scientists and science lovers anywhere!
Science commits suicide when it adopts a creed. ~Thomas Henry Huxley
Link Details
| ID: | 1313 |
| Title: | Protocol for Transcriptional Run- On Assays - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3956 |
| Description: | In this protocol nuclei isolated from cells expressing the gene of interest are incubated with radiolabeled UTP which is incorporated into nascent RNA transcripts by RNA polymerase molecules that were actively transcribing at the time the cells were harvested. Because very little denovo initiation of RNA synthesis occurs in isolated nuclei, transcription of the target gene can be measured by hybridizing the radiolabeled RNA to an excess of the target gene immobilized on a nitrocellulose or nylon |
| Category: | Cell Biology and Cell Culture: In Vitro Reconstitution Protocols |
| Link Owner: | |
| Date Added: | November 27, 2007 02:21:40 AM |
| Number Hits: | 6 |
Send this page to a friend