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Protocol for Transcriptional Run- On Assays - Details

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Protocols » Listing Details
ID:1313
Title: Protocol for Transcriptional Run- On Assays - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3956
Description:In this protocol nuclei isolated from cells expressing the gene of interest are incubated with radiolabeled UTP which is incorporated into nascent RNA transcripts by RNA polymerase molecules that were actively transcribing at the time the cells were harvested. Because very little denovo initiation of RNA synthesis occurs in isolated nuclei, transcription of the target gene can be measured by hybridizing the radiolabeled RNA to an excess of the target gene immobilized on a nitrocellulose or nylon
Category:Cell Biology and Cell Culture: In Vitro Reconstitution Protocols
Link Owner:
Date Added:November 27, 2007 02:21:40 AM
Number Hits:17
 

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