Bioinformatics, Protocols, DNA RNA Protein Proteomics
home > protocol-links > detail.php
 |
|---|
You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!
Already registered? Login now below.
Already registered and Forgot your password? Click below to recover it.
Join now - it's fast and free!
Molecular Station is THE largest network of researchers, scientists and science lovers anywhere!
Put off your imagination, as you put off your overcoat, when you enter the laboratory. But put it on again, as you put on your overcoat, when you leave. ~Attributed to Claude Bernard (French physiologist, 1813-1878)
Link Details
| ID: | 1306 |
| Title: | Protocol for Polymerase III In Vitro Transcription - http://www.bio.com/protocolstools/protocol.jhtml?id=p1140 |
| Description: | Protocol describes an RNA Polymerase III (Pol III) transcription assay using an extract or proteins of choice. Pol III is the polymerase responsible for transcribing 5S RNA, tRNAs, and other small RNAs. α-Amanitin inhibits Pol II transcription in the assay. The newly-transcribed, radiolabeled RNA is visualized by autoradiography following Urea Polyacrylamide gel electrophoresis. |
| Category: | Cell Biology and Cell Culture: In Vitro Reconstitution Protocols |
| Link Owner: | |
| Date Added: | September 24, 2007 01:41:17 PM |
| Number Hits: | 9 |
Send this page to a friend