Bioinformatics, Protocols, DNA RNA Protein Proteomics
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Link Details
| ID: | 1301 |
| Title: | In Vitro Transcription Assay (Run-off Assay) using Permeabilized Cells - http://www.bio.com/protocolstools/protocol.jhtml?id=p1971 |
| Description: | With this protocol, transcripts that were initiated from specific genes by RNA polymerases prior to permeabilization can be measured. Instead of a nuclear extract, permeabilized cells are used. Includes information on: Permeabilization of Cells; In vitro Transcription Reaction (Run-off); Isolation of RNA; Preparation of Slot Blot Membrane for Hybridization; Hybridization of Nitrocellulose Membrane; TCA Precipitation to Determine Incorporation of [32P] GTP into Nucleic Acid |
| Category: | Cell Biology and Cell Culture: In Vitro Reconstitution Protocols |
| Link Owner: | |
| Date Added: | September 24, 2007 08:55:30 AM |
| Number Hits: | 50 |
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