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In Vitro Transcription Assay (Run-off Assay) using Permeabilized Cells - Details

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Protocols » Listing Details
ID:1301
Title: In Vitro Transcription Assay (Run-off Assay) using Permeabilized Cells - http://www.bio.com/protocolstools/protocol.jhtml?id=p1971
Description:With this protocol, transcripts that were initiated from specific genes by RNA polymerases prior to permeabilization can be measured. Instead of a nuclear extract, permeabilized cells are used. Includes information on: Permeabilization of Cells; In vitro Transcription Reaction (Run-off); Isolation of RNA; Preparation of Slot Blot Membrane for Hybridization; Hybridization of Nitrocellulose Membrane; TCA Precipitation to Determine Incorporation of [32P] GTP into Nucleic Acid
Category:Cell Biology and Cell Culture: In Vitro Reconstitution Protocols
Link Owner:
Date Added:September 24, 2007 08:55:30 AM
Number Hits:160
 

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