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In vitro Translation Assays for CFTR - Details

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Protocols » Listing Details
ID:1297
Title: In vitro Translation Assays for CFTR - http://pen2.igc.gulbenkian.pt/cftr/vr/c/ostedgaard_in_vitro_translation_assays_for_cftr.pdf
Description:Protocol outlines the general procedure and requirements for in vitro translation of CFTR and outlines some assays using in vitro translated product. Core glycosylation of CFTR occurs in the ER. An assay for this processing step requires the addition of microsomal membranes to the basic in vitro translation mixture. This protocol takes this into account.
Category:Cell Biology and Cell Culture: In Vitro Reconstitution Protocols
Link Owner:
Date Added:September 24, 2007 08:21:52 AM
Number Hits:35
 

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