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Analysis of Membrane Trafficking and Intracellular Signaling in Self-Generated Iodixanol Gradients - Details

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Protocols » Listing Details
ID:1287
Title: Analysis of Membrane Trafficking and Intracellular Signaling in Self-Generated Iodixanol Gradients - http://www.axis-shield.com/densityhome/optiprep/S25.pdf
Description:Protocol is based on methods for the resolution of GLUT4 containing vesicles and the identification of phosphoinositide kinase containing vesicles in 3T3-L1 adipocytes. They may have a wider application to any low-medium density membranes. Protocol incorporates the strategy of using a low density microsome fraction as the gradient input, commonly used in GLUT 4 studies that may have a wider application to other investigations.
Category:Protein Protocols: Protein Trafficking
Link Owner:
Date Added:September 18, 2007 05:00:40 AM
Number Hits:128
 

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