Protocol is based on methods for the resolution of GLUT4
containing vesicles and the identification of phosphoinositide kinase containing vesicles in 3T3-L1 adipocytes. They may have a wider application to any low-medium density membranes. Protocol incorporates the strategy of using a low density microsome fraction as the gradient input, commonly
used in GLUT 4 studies that may have a wider application to other investigations.