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General Method for Ras, Rac, Cdc42, and Rho Activation Assay - Details

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Protocols » Listing Details
ID:1278
Title: General Method for Ras, Rac, Cdc42, and Rho Activation Assay - http://www.upstate.com/misc/protocol_detail.q.prot.e.general_method_ras.a.name.e.General_Method_for_Ras_Rac_Cdc42_and_Rho_Activation_Assay
Description:General protocol for Ras, Rac, Cdc42, and Rho activation assay. Includes: Affinity Precipitation/Immunoblot Protocol, Cell Culture and Extract Preparation (Adherent and Non Adherent cells), GTPγS/GDP Loading for Positive and Negative Controls, Ras, Rac ,Cdc42, and Rho Pull-Down Assay and Western Blot and Detection.
Category:Signalling Signal Transduction Protocols
Link Owner:
Date Added:September 18, 2007 03:14:54 AM
Number Hits:163
 

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