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Protocol for Competitive Ligand Binding to Cortical Receptor using Crosslinking - Details

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Protocols » Listing Details
ID:1276
Title: Protocol for Competitive Ligand Binding to Cortical Receptor using Crosslinking - http://www.bio.com/protocolstools/protocol.jhtml?id=p1254
Description:Protocol describes a competitive ligand binding assay for cortical neurotrophin receptors. Following binding in the presence of competitor, the bound radiolabeled ligand is cross-linked to the receptor. The cells are lysed and the ligand-receptor complexes are immunoprecipitated using a pan-trk (tyrosine kinase receptor) antibody. Protocol includes:Preparation of Cortical Tissue for Competitive Crosslinking, Competitive Binding, Crosslinking Ligand to Receptor, Lysis and Immunoprecipitation etc
Category:Signalling Signal Transduction Protocols
Link Owner:
Date Added:September 18, 2007 01:16:53 AM
Number Hits:18
 

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