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Protocol for Antiphosphotyrosine Western Blot Analysis - Details

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Protocols » Listing Details
ID:1275
Title: Protocol for Antiphosphotyrosine Western Blot Analysis - http://www.bio.com/protocolstools/protocol.jhtml?id=p697
Description:Detection of phosphorylated tyrosine residues can be performed using anti-P-TYR Ab and Western Analysis.Includes 2nd method,which uses phosphotyrosine in conjunction with anti-P-TYR Ab to "unlabel" potential proteins.By comparing Westerns developed with the 1st method(reveals phosphorylated protein) and the 2nd method(reveals non-specific labeling), a more accurate picture of those proteins phosphorylated on tyrosine can be seen. Includes: Protein Preparation, Electrophoresis and Transfer.
Category:Signalling Signal Transduction Protocols
Link Owner:
Date Added:September 18, 2007 01:12:37 AM
Number Hits:20
 

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