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Preparation of RAW 264.7 Lysates for Cyclic AMP Determination - Details

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Protocols » Listing Details
ID:1264
Title: Preparation of RAW 264.7 Lysates for Cyclic AMP Determination - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000175.pdf?pid=PP00000175
Description:The protocol provides a method to achieve a sample sufficient for one determination of cAMP using the acetylation protocol. The protocol describes the method used for measuring the content of cyclic adenosine 3',5'- monophosphate (cyclic AMP or cAMP) in RAW 264.7 cells using an enzyme-linked immunoassay system. Information included in the protocol: Treatment of Cells and Preparation of Extracts; Reagents and Materials.
Category:Signalling Signal Transduction Protocols
Link Owner:
Date Added:September 14, 2007 12:20:38 PM
Number Hits:10
 

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