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GTPγS-Induced Inhibition of cAMP Binding to the cAMP Receptor (cAR1) in Dictyostelium Discoideum - Details

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Protocols » Listing Details
ID:1260
Title: GTPγS-Induced Inhibition of cAMP Binding to the cAMP Receptor (cAR1) in Dictyostelium Discoideum - http://www.bio.com/protocolstools/protocol.jhtml?id=p1175
Description:This protocol assays inhibition of in vivo binding of [3H]-cAMP to cAR1 by GTPγS. Dictyostelium discoideum respond to extracellular cAMP through the cAMP chemoattractant receptor (cAR1). Binding of cAMP to the G protein-coupled cAR1 is inhibited by the GTP analog GTPγS. Protocol includes information on: Solutions used, BioReagents and Chemicals and Protocol Hints.
Category:Signalling Signal Transduction Protocols
Link Owner:
Date Added:September 14, 2007 10:45:53 AM
Number Hits:8
 

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