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Assay of Cyclic AMP in Lysates of Cells - Details

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Protocols » Listing Details
ID:1259
Title: Assay of Cyclic AMP in Lysates of Cells - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000015.pdf?pid=PP00000015
Description:Protocol allows you to measure the content of cyclic adenosine 3',5'-monophosphate (cyclic AMP or cAMP) in splenic B lymphocytes (B cells) in an enzyme-linked immunoassay. This protocol utilizes acetylation of cAMP to improve sensitivity and reduce interference. Protocol includes information on: how to determine cAMP, calculations and reagents and materials.
Category:Signalling Signal Transduction Protocols
Link Owner:
Date Added:September 14, 2007 10:43:08 AM
Number Hits:58
 

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