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Assay of Intracellular Free Calcium in RAW 264.7 Cells Loaded with Fura-2 (with FLEXstation) - Details

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Protocols » Listing Details
ID:1241
Title: Assay of Intracellular Free Calcium in RAW 264.7 Cells Loaded with Fura-2 (with FLEXstation) - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000211.pdf?pid=PP00000211
Description:This protocol describes a method to assess concentrations of free cytoplasmic calcium, [Ca2+], in cultured adherent RAW 264.7 cells, using a 96-well plate format. This objective is accomplished by using the Ca2+-sensitive fluorescent dye, fura-2, which permeates cell membranes as an ester and is hydrolyzed in the cell to its Ca2+-sensitive acidic form.
Category:Signalling Signal Transduction Protocols: Calcium Measurement Protocols
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Date Added:September 07, 2007 10:58:24 AM
Number Hits:146
 

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