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Assay of Intracellular Free Calcium in RAW 264.7 Cells - Details

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Protocols » Listing Details
ID:1239
Title: Assay of Intracellular Free Calcium in RAW 264.7 Cells - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000210.pdf?pid=PP00000210
Description:This protocol describes a method to assess concentrations of free cytoplasmic calcium, [Ca2+]i, in cultured adherent RAW 264.7 cells, using a 96-well plate format. This objective is accomplished by using the Ca2+-sensitive fluorescent dye, fluo-3, which permeates cell membranes as an ester and is hydrolyzed in the cell to its Ca2+-sensitive acidic form.
Category:Signalling Signal Transduction Protocols: Calcium Measurement Protocols
Link Owner:
Date Added:September 07, 2007 10:56:00 AM
Number Hits:311
 

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