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ANTIBODY PURIFICATION by affinity chromatography - Details

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Protocols » Listing Details
ID:1235
Title: ANTIBODY PURIFICATION by affinity chromatography - http://mullinslab.ucsf.edu/protocols/html/ANTIBODY_PURIFICATION_by_affini.htm
Description:ANTIBODY PURIFICATION by affinity chromatography. By Beth, Mullins Lab UCSF. To affinity purify antibodies, generate lots of E. coli lysate that contains your antigen. If the protein can stand freeze thawing, then go ahead and purify the protein from e. coli lysate and keep it frozen until you need to couple it to a CH-sepharose column.
Category:Chromatography Protocols: Immunoaffinity Chromatography Protocols
Link Owner:
Date Added:September 07, 2007 10:49:08 AM
Number Hits:368
 

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